Therefore, the purpose of the present study was to investigate the activity and the isoenzyme profile of these two key enzymes of myocardial energy metabolism in rabbits with left ventricular LV dysfunction due to repetitive treatment with high doses of catecholamines. This infusion was repeated at days 12, 24, and LV dysfunction was associated with a decreased total LV lactate dehydrogenase activity LDH; 0. Box Beaumont Texas. See all Haemostasis See all Molecular products Xk manufacturers and suppliers of clinical diagnostic equipment, software and assays for over thirty years.



For full functionality of ResearchGate it is necessary to enable JavaScript. Eufope demonstrated a significant deterioration of LV function as well as increased LV-diameter and -mass index in catecholamine-induced cardiomyopathy. Histological examination revealed that repetitive catecholamine infusion resulted in LV fibrous areas with collagenous content and an increase sioenzyme myocyte width LV dysfunction was associated with a decreased total LV lactate dehydrogenase activity LDH; 0.

Furthermore, myocardial LDH isoenzymes were shifted with a decrease in LDH 1 and an increase in LDH2 and LDH3 LDH 1 : Foetal B-CK isoenzymes were significantly increased CK-MB 5. The current study demonstrates changes in cardiac energy metabolism including an impaired LDH activity isoejzyme a shift towards anaerobic isoenzymes as well as a more efficient CK system in a model of catecholamine-induced LV dysfunction.

The purpose of the current study was to evaluate myocardial creatinine kinase CK and lactate dehydrogenase Isoenzymf systems in a model of epinephrine-induced cardiomyopathy in rabbits. Furthermore, myocardial LDH isoenzymes were shifted with a decrease in LDH and an increase in LDH and LDH LDH : Such changes may be also noticed in patients treated with high doses of catecholamines or with pheochromocytoma, which Corresponding author.

Isoenzmye and creatinine kinase CK hleena not been studied. As we have previously demon- strated, this pdf helena biosciences titan gel ck ld isoenzyme europe of LV dysfunction is associated with markedly impaired cardiac function, neurohu - moral activation, as well as systemic and local vaso- constriction 6. Sixteen male Chinchilla rabbits 3.

This infusion was isonezyme at days 12, 24, and Thereafter, rabbits were killed and the hearts quickly excised to study energy metabolism and histological changes after treatment wit h epinephri ne. Eight rabbits without cate- cholamine-treatment served as controls CTRL. Planimetric measurements were carried out to de- termine areas of LV chamber as well as septum and LV free wall. For histological evaluation, three tissue slices from each heart were embedded after dehydra- tion and cleaning.

Sections were graded and averaged to produce a single score for the entire Myocyte width was determined by computer- assisted quantitative image analysis Olympus Optical Corp. In randomly assigned slides only myocytes per with a visible nucleus were selected and the distance between the borders were measured average of myocytes per slide. The histological examination was per- formed by two independent heleena who were blinded with respect to administration of epinephrine.

Myocardial enzyme measurements LV tissue samples were homogenised in 0. Aliquots were taken to measure protein content by the method of Lowry For the measurement of CK isoenzyme distribu - tion the Rapid Electrophoresis System REP, Helena Diagnostik GmbH was used as surope unit, pdf helena biosciences titan gel ck ld isoenzyme europe the REP CK isoforms kit Helena Diagnostika GmbH for agarose gel and incubation solution were applied.





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